專長
RNA Biology、miRNA調控機制、mRNA降解機制

辦公室
生物醫學大樓 五樓 R505 室

電話
886-2-2826-7122

Email
chungte.chang@nycu.edu.tw

個人網頁
GO

ORCiD
0000-0002-4792-1646

學經歷

2019 迄今
2012-2019
2008-2012

國立陽明大學 生化暨分子生物研究所 助理教授
德國 馬克思普朗克發育生物學研究所 博士後研究員
英國 雪菲爾大學 博士

榮譽

2021
2020
2019

國立陽明大學良師益友導師
國立陽明大學良師益友導師
科技部補助大專校院延攬特殊優秀人才

研究方向

在過去,基因表達(gene expression)一般被認為主要是受到轉錄水平(transcriptional level)的調控。然而,由於RNA沉默(RNA silencing)機制的發現 ,以及在轉錄後調控(Post-transcriptional regulation)領域的研究,使人們認識 到轉錄後調控機制提供了細胞得以迅速改變基因表達的模式。在基因轉錄後的 過程中(例如: mRNA processing,export,surveillance,silencing和turnover), 藉由使用各種調控因子的相互連結,構成了極為複雜的調節網絡,使細胞具有 面對外在環境變化的快速應變能力。

為了能進一步了解基因轉錄後調控的分子機制,本實驗室的主要目標為:

1.利用生化及分生的方法,為各種轉錄後調控因子建立一個相互作用的網絡列表,以深入解析他們是如何相互聯繫及調控;
2.使用CRISPR/Cas9系統剃除特定的調控因子,藉此了解其對轉錄後調控機制以及特定基因表達的重要性;
3.藉由次世代定序(Next-Generation Sequencing)來深入分析當剃除特定的調控 因子後,內源性基因的調節與人類疾病的相關性。

研究著作

Enwerem III, Elrod ND, Chang CT, Lin A, Ji P, Bohn JA, Levdansky Y, Wagner EJ, Valkov E, Goldstrohm AC. (2021) Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome. RNA. 27:445-464.

Arvola RM, Chang CT, Buytendorp JP, Levdansky Y, Valkov E, Freddolino PL, Goldstrohm AC. (2020) Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs. Nucleic Acids Res. 48:1843-1871.

Hanet A, Räsch F, Weber R, Ruscica V, Fauser M, Raisch T, Kuzuoğlu-Öztürk D, Chang CT, Bhandari D, Igreja C, Wohlbold L. (2019) HELZ directly interacts with CCR4-NOT and causes decay of bound mRNAs. Life Sci Alliance. 2:e201900405.

Chang CT, Muthukumar S, Weber R, Levdansky Y, Chen Y, Bhandari D, Igreja C, Wohlbold L, Valkov E, Izaurralde E. (2019) A low-complexity region in human XRN1 directly recruits deadenylation and decapping factors in 5′-3′ messenger RNA decay. Nucleic Acids Res. [Epub ahead of print]

Raisch T*,Chang CT*, Levdansky Y*, Muthukumar S, Raunser S, Valkov E. (2019) Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation. Nat Commun. 10:3173. (*co-first)

Valkov E*, Muthukumar S*, Chang CT*, Jonas S, Weichenrieder O, Izaurralde E. (2016) Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Nat Struct Mol Biol. 23:574-9. (*co-first)

Chen Y, Boland A, Kuzuoğlu-Öztürk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E. (2014) A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Mol Cell. 54:737-50.

Chang CT, Bercovich N, Loh B, Jonas S, Izaurralde E. (2014) The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1. Nucleic Acids Res. 42:5217-33.

Chang CT*, Hautbergue GM*, Walsh MJ, Viphakone N, van Dijk TB, Philipsen S, Wilson SA. (2013) Chtop is a component of the dynamic TREX mRNA export complex. EMBO J. 32:473-86. (*co-first)

Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, Weichenrieder O, Coles M, Izaurralde E. (2012) A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5′ exonucleolytic degradation. Nat Struct Mol Biol. 19:1324-31.

Viphakone N, Hautbergue GM, Walsh M, Chang CT, Holland A, Folco EG, Reed R, Wilson SA. (2012) TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export. Nat Commun. 3:1006.

Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang CT, Dickman MJ, Essex-Lopresti A, Harding SV, Mahadi NM, Marshall LE, Mobbs GW, Mohamed R, Nathan S, Ngugi SA, Ong C, Ooi WF, Partridge LJ, Phillips HL, Raih MF, Ruzheinikov S, Sarkar-Tyson M, Sedelnikova SE, Smither SJ, Tan P, Titball RW, Wilson SA, Rice DW. (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science. 334:821-4.

Hautbergue GM, Hung ML, Walsh MJ, Snijders AP, Chang CT, Jones R, Ponting CP, Dickman MJ, Wilson SA. (2009) UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA. Curr Biol. 19:1918-24.

Su MC, Chang CT, Chu CH, Tsai CH, Chang KY. (2005) An atypical RNA pseudoknot stimulator and an upstream attenuation signal for -1 ribosomal frameshifting of SARS coronavirus. Nucleic Acids Res. 33:4265-75.