經歷

榮譽

指導學生獲獎

研究方向

(1) Stimuli-Responsive DNA Microcapsules：We develop a method to synthesize substrate-loaded DNA microcapsules acting as drug carriers and being unlocked by stimuli. The base pairing of nucleotides provides a means to assemble microcapsules by interlinking DNA layers via hybridization. By programming the base sequences of DNA, stimuli-responsive functionalities encoded in the DNA shell of microcapsules allow for the dissociation of microcapsules in the presence of appropriate triggers and for the release of drugs.

(2) Nucleoapzymes：The concept of nucleoapzymes is to improve the catalytic functions of nucleic acids and to develop versatile applications in DNA nanotechnology. The nucleoapzyme is a hybrid structure of nucleic acids by the conjugation of a substrate binding aptamer with a DNAzyme sequence to mimic a native enzyme with both a substrate binding site and an active site. We study the catalytic activity of nucleoapzymes with different orientation and distance between the aptamer and DNAzyme units and find their activities can be controlled by their configurations.

(3) Biosensors：To achieve a rapid and accurate detection of analytes, we develop various sensing platforms. Specifically, we utilize biomolecules (e.g., nucleic acids, antibodies) as recognition elements to bind analytes and introduce signal amplification mechanisms (e.g., nanozymes, enzyme cascades) to convert the analyte information to optical or electrical output signals.

研究著作

Huang, L.-H.; Hsieh, Y.-Y.; Yang, F.-A.; Liao, W.-C.* DNA-modified Prussian Blue Nanozymes for Enhanced Electrochemical Biosensing. Nanoscale 2024, 16, 9770-9780.

Singuru, M. M. R.; Chen, J.-L.; Chen, H.-Y.;Liao, W.-C.; Chen, Y.-Y.; Chuang, M.-C. MercuryII-Mediated Construction of DNA Capsules for Turn-On Fluorescence Detection of Melamine. Microchim. Acta 2024, 191, 658.

Lin, Y.-H.; Singuru, M. M. R.; Marpaung, D. S. S.; Liao, W.-C.*; Chuang, M.-C.* Ethylene Glycol-Manipulated Syntheses of Calcium Carbonate Particles and DNA Capsules toward Efficient ATP-Responsive Cargo Release. ACS Appl. Bio Mater. 2023, 6, 3351-3360.

Yang, F.-A.; Wu, Y.-T.; Liu, Y.-W.; Liao, W.-C.* Hybridization Chain Reaction-Assisted Enzyme Cascade Genosensor for the Detection of Listeria monocytogenes. Talanta 2023, 254, 124193.

Singuru, M. M. R.; Liao, Y.-C.; Lin, G. M.-H.; Chen, W.-T.; Lin, Y.-H.; To, C. T.; Liao, W.-C.; Hsu, C.-H.; Chuang, M.-C.* Engineered Multivalent DNA Capsules for Multiplexed Detection of Genotoxicants via Versatile Controlled Release Mechanisms. Biosens. Bioelectron. 2022, 216, 114608.

Chang, W.-H.; Lee, Y.-F.; Liu, Y.-W.; Willner, I.*; Liao, W.-C.* Stimuli-Responsive Hydrogel Microcapsules for the Amplified Detection of MicroRNAs. Nanoscale 2021, 13, 16799-16808.

Singuru, M. M. R.; Liao, W.-C.*; Chuang, M.-C.* Genotoxin-Responsive DNA Capsules for the Detection of Styrene Oxide. Sensors Actuators B: Chem. 2021, 349, 130776.

Lilienthal, S.; Fischer, A.; Liao, W.-C.; Cazelles, R.; Willner, I.* Single and Bilayer Polyacrylamide Hydrogel-Based Microcapsules for the Triggered Release of Loads, Logic Gate Operations, and Intercommunication between Microcapsules. ACS Appl. Mater. Interfaces 2020, 12, 31124–31136.

Chen, W.-H.; Liao, W.-C.; Sohn, Y. S.; Fadeev, M.; Cecconello, A.; Nechushtai, R.; Willner, I.* Stimuli-Responsive Nucleic Acid-Based Polyacrylamide Hydrogel-Coated Metal–Organic Framework Nanoparticles for Controlled Drug Release. Adv. Funct. Mater. 2018, 28, 1705137.

Liao, W.-C.; Willner, I. Synthesis and Applications of Stimuli-Responsive DNA-Based Nano- and Micro-Sized Capsules. Adv. Funct. Mater. 2017, 27, 1702732.

Chen, W.-H.; Yu, X.; Liao, W.-C.; Sohn, Y. S.; Cecconello, A.; Kozell, A.; Nechushtai, R.; Willner, I.* ATP-Responsive Aptamer-Based Metal–Organic Framework Nanoparticles (NMOFs) for the Controlled Release of Loads and Drugs. Adv. Funct. Mater. 2017, 27, 1702102.

Liao, W.-C.; Lilienthal, S.; Kahn, J. S.; Riutin, M.; Sohn, Y. S.; Nechushtai, R.; Willner, I.* pH- and Ligand-Induced Release of Loads from DNA-Acrylamide Hydrogel Microcapsules. Chem. Sci. 2017, 8, 3362-3373.

Wang, S.; Cazelles, R.; Liao, W.-C.; Vázquez-González, M.; Zoabi, A.; Abu-Reziq, R.; Willner, I.* Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu2+-Modified Graphene Oxide Nanoparticles. Nano Lett. 2017, 17, 20432048.

Vázquez-González, M.; Liao, W.-C.; Cazelles, R.; Wang, S.; Yu, X.; Gutkin, V.; Willner, I.* Mimicking Horseradish Peroxidase Functions Using Cu2+-Modified Carbon Nitride Nanoparticles or Cu2+-Modified Carbon Dots as Heterogeneous Catalysts. ACS Nano 2017, 11, 32473253.

Liao, W.-C.; Riutin, M.; Parak, W. J.; Willner, I.* Programmed pH-Responsive Microcapsules for the Controlled Release of CdSe/ZnS Quantum Dots. ACS Nano 2016, 10, 8683–8689. Highlighted on nanotechweb.org, Aug 29, 2016. pH-Responsive DNA Capsules Release QD Loads, available at: http://nanotechweb.org/cws/article/tech/66042

Liao, W.-C.; Sohn, Y. S.; Riutin, M.; Cecconello, A.; Parak, W. J.; Nechushtai, R.; Willner, I.* The Application of Stimuli-Responsive VEGF- and ATP-Aptamer-Based Microcapsules for the Controlled Release of an Anticancer Drug, and the Selective Targeted Cytotoxicity toward Cancer Cells. Adv. Funct. Mater. 2016, 26, 4262–4273.

Huang, F.; Liao, W.-C. (equal contribution); Sohn, Y. S.; Nechushtai, R.; Lu, C.-H.; Willner, I.* Light-Responsive and pH-Responsive DNA Microcapsules for Controlled Release of Loads. J. Am. Chem. Soc. 2016, 138, 8936–8945.

Golub, E.; Albada, H. B.; Liao, W.-C.; Biniuri, Y.; Willner, I.* Nucleoapzymes: Hemin/G-Quadruplex DNAzyme–Aptamer Binding Site Conjugates with Superior Enzyme-like Catalytic Functions. J. Am. Chem. Soc. 2016, 138, 164–172.

Liao, W.-C.; Lu, C.-H.; Hartmann, R.; Wang, F.; Sohn, Y. S.; Parak, W. J.*; Willner, I.* Adenosine Triphosphate-Triggered Release of Macromolecular and Nanoparticle Loads from Aptamer/DNA-Cross-Linked Microcapsules. ACS Nano 2015, 9, 9078–9086. Highlighted on nanotechweb.org, Aug 24, 2015. Cross-Linked Microcapsules Release Anti-Cancer Drugs, available at: http://nanotechweb.org/cws/article/tech/62303

Lin, C.-H.*; Liao, W.-C.; Chen, H.-K.; Kuo, T.-Y. Paper Spray-MS for Bioanalysis. Bioanalysis 2014, 6, 199–208.

Liao, W.-C.; Ho, J.-a. A.* Improved Activity of Immobilized Antibody by Paratope Orientation Controller: Probing Paratope Orientation by Electrochemical Strategy and Surface Plasmon Resonance Spectroscopy. Biosens. Bioelectron. 2014, 55, 32–38.

Liao, W.-C.; Chuang, M.-C.; Ho, J.-a. A.* Electrochemical Sensor for Multiplex Screening of Genetically Modified DNA: Identification of Biotech Crops by Logic-Based Biomolecular Analysis. Biosens. Bioelectron. 2013, 50, 414–420.

Ho, J.-a. A.*; Hsu, W.-L.; Liao, W.-C.; Chiu, J.-K.; Chen, M.-L.; Chang, H.-C.; Li, C.-C. Ultrasensitive Electrochemical Detection of Biotin Using Electrically Addressable Site-Oriented Antibody Immobilization Approach via Aminophenyl Boronic Acid. Biosens. Bioelectron. 2010, 26, 1021–1027.

Liao, W.-C.; Ho, J.-a. A.* Attomole DNA Electrochemical Sensor for the Detection of Escherichia coli O157. Anal. Chem. 2009, 81, 2470–2476.

Cheng, Y. J.; Liao, W.-C.; Wang, C. M.* Proton-Assisted Iron(III) Recognition with 4,4’-(4,5-Diaminonaphthalen-1,8-Yldiazo)-Bisbenzenesulfonate. J. Electroanal. Chem. 2008, 612, 15–21.

Viswanathan, S.; Liao, W.-C.; Huang, C.-C.; Hsu, W.-L.; Ho, J.-a. A.* Rapid Analysis of L-Dopa in Urine Samples Using Gold Nanoelectrode Ensembles. Talanta 2007, 74, 229–234.