經歷

榮譽

指導學生獲獎

專利與技轉

專利名稱：製備改良豬血漿纖連接蛋白以增強傷口癒合之應用
發明人：Jean-Cheng Kuo (郭津岑)
中華民國發明專利證號：發明第I674903號 (專利期間: 2019年10月21日~2037年09月21日)
技轉：嘉生細胞科技股份有限公司 (2016年11月-2032年10月)

專利名稱：Process for a preparation of the modified porcine plasma fibronectin for enhance wound healing
發明人：Jean-Cheng Kuo (郭津岑)
美國發明專利案號 (US patent NO.)：US 10,590,184 B2 (專利期間: 2020年03月17日~2038年01月03日)
技轉：嘉生細胞科技股份有限公司 (2016年11月-2032年11月)

專利名稱：Process for a preparation of the modified porcine plasma fibronectin for enhance wound healing
發明人：Jean-Cheng Kuo (郭津岑)
美國發明專利案號(分割案)：16/779300 (申請日期：2020.1.31)
技轉：嘉生細胞科技股份有限公司 (2016年11月-2032年11月)

研究方向

(一) 幹細胞分化及癌症轉移的研究：細胞在生理微環境中透過黏著斑 (focal adhesions)的形成與細胞外間質(extracellular matrix)貼附，以感受並傳遞細胞外間質的生化及物理性刺激(包括: 細胞外間質組成、硬度、孔隙大小及形狀)，進而轉化為細胞內的生物信號，以調節細胞各式生理功能(包括:細胞分裂、分化、細胞移動及凋亡)。本實驗室透過各種模擬人體條件的狀況下，結合蛋白質體學分析及高階顯微鏡技術以探討間葉幹細胞分化及癌細胞轉移的調控機轉及應用。

Stem cell differentiation and cancer metastasis: We focus on the topic of the mechanosensitivity of integrin-based focal adhesions, which allow cells to sense and translate biochemical and physical cues from their environment (extracellular matrix components, stiffness or organization) into cellular biological signals that control cell behavior, differentiation, and death. My group focus on investigating how the signals of integrin-based focal adhesions regulate stem cell fate, myogenesis and cancer metastasis.

(二)傷口癒合材料的開發：纖維連接蛋白(fibronectin)為已知促進細胞移動的細胞外間質蛋白，本實驗室開發纖維連接蛋白純化之專利技術，將持續開發為傷口癒合材料。

Development of wound healing materials: We have developed a patented method to purify fibronectin. Using the purified fibronectin, my group keep developing the medical materials for wound healing.

研究著作

Wu MC, Yu HW, Chen YQ, Ou MH, Serrano R, Huang GL, Wang YK, Lin KH, Fan YJ, Wu CC, del Álamo JC, Chiou A, Chien S, Kuo JC^§. Early committed polarization of intracellular tension in response to cell shape determines the osteogenic differentiation of mesenchymal stromal cells. Acta Biomaterialia. (in press) 2022.

Chiu SH, Ho WL, Sun YC, Kuo JC, Huang JR^§. Phase separation driven by interchangeable properties in the intrinsically disordered regions of protein paralogs. Communication Biology. 29;5(1):400. 2022.

Lin JW, Huang YM, Chen YQ, Chuang TY, Lan TY, Liu YW, Pan HW, You LR, Wang YK, Lin KH, Chiou A, Kuo JC^§. Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals. Cell Death Discov. 7(1):35. 2021.

Chen YQ^§, Kuo JC, Wei MT, Wu MC, Yang MH, Chiou A^§. Fibroblast Promotes Head and Neck Squamous Cell Carcinoma Cell Invasion through Mechanical Barriers in 3D Collagen Microenvironments. ACS Applied Bio Materials. 3, 6419−6429. 2020.

Le Saux G, Wu MC, Toledo E, Chen YQ, Fan YJ, Kuo JC^§, Schvartzman M^§. Cell-Cell Adhesion-Driven Contact Guidance and Its Effect on Human Mesenchymal Stem Cell Differentiation. ACS Applied Materials & Interfaces. 20;12(20):22399-22409. 2020.

Chiu YP, Sun YC, Qiu DC, Lin YH, Chen YQ, Kuo JC, Huang JR^§. Liquid-liquid Phase Separation and Extracellular Multivalent Interactions in the Tale of galectin-3. Nature Communication. 6;11(1):1229. 2020.

Cheng HW, Hsiao CT, Chen YQ, Huang CM, Chan SI, Chiou A, Kuo JC^§. Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration. Life Sci Alliance. 8; 2(1). 2019.

Huang KC, Wang ML, Chen SJ, Kuo JC, Wang WJ, Nhi Nguyen PN, Wahlin KJ, Lu JF, Tran AA, Shi M, Chien Y, Yarmishyn AA, Tsai PH, Yang TC, Jane WN, Chang CC, Peng CH, Schlaeger TM, Chiou SH^§. Morphological and Molecular Defects in Human Three-Dimensional Retinal Organoid Model of X-Linked Juvenile Retinoschisis. Stem Cell Reports. 13(5):906-923. 2019.

Chen YQ§, Kuo JC, Wei MT, Chen YC, Yang MH, Chiou A^§. Early stage mechanical remodeling of collagen surrounding head and neck squamous cell carcinoma spheroids correlates strongly with their invasion capability. Acta Biomaterialia. 15; 84:280-292. 2019.

Luo R, Chen PW, Kuo JC, Jenkins L, Jian X, Waterman CM, Randazzo PA^§. ARAP2 inhibits Akt independently of its effects on focal adhesions. Biol Cell. 110(12): 257-270. 2018.

Hsiao CT, Cheng HW, Huang CM, Lee HR, Ou MH, Huang JR, Khoo KH, Yu HW, Chen YQ, Wang YK, Chiou A, Kuo JC^§. Fibronectin in cell adhesion and migration via N-glycosylation. Oncotarget 8 (41): 70653-70668. 2017.

Chuang TY, Cheng AJ, Lan TY, Huang IH, Shiau CW, Hsu CL, Liu YW, Tseng PH, Chang ZF, Kuo JC^§. Suppression of LPS-induced inflammatory responses by the hydroxyl groups of dexamethasone. Oncotarget 8: 49735-49748. 2017.

Chakraborty S, Ou MH, Kuo JC^§, Chiou A^§. A comparative study of metabolic state of stem cells during osteogenic and adipogenic differentiations via fluorescence lifetime imaging microscopy. Proc. of SPIE Vol. 10024:100243Y. 2016.

Wu TH, Kuo YY, Lee HH, Kuo JC, Ou MH, Chang ZF^§. Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization. Sci. Rep. 6:28685. 2016.

Wu JC, Chen YC, Kuo CT, Wenshin Yu H, Chen YQ, Chiou A, Kuo JC^§. Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration. Sci. Rep. 5:18476. 2015.

Wang HL, Yang CH, Lee HH, Kuo JC, Hur SS, Chien S, Lee OK, Hung SC, Chang ZF^§. Dexamethasone-induced cellular tension requires SGK1-stimulated Sec5/GEF-H1 interaction. J Cell Sci. 128(20):3757-68. 2015.

Yu HW, Chen YQ, Huang CM, Liu CY, Chiou A, Wang YK, Tang MJ, Kuo JC^§. β-PIX controls intracellular viscoelasticity to regulate lung cancer cell migration. J Cell Mol Med. 19(5): 934-947. 2015.

Huang IH, Hsiao CT, Wu JC, Shen RF, Liu CY, Wang YK, Chen YC, Huang CM, del Álamo JC, Chang ZF, Tang MJ, Khoo KH, Kuo JC^§. GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment. J Cell Sci. 127:4186-4200. 2014.* This paper was selected to be the main Journal of Cell Science October cover.

Kuo JC^§. Focal adhesions function as a mechanosensor. Progress in Molecular Biology and Translational Science. 126:55-73. 2014.

Yuan WC, Lee YR, Lin SY, Chang LY, Tan YP, Hung CC, Kuo JC, Liu CH, Lin MY, Xu M, Chen ZJ, Chen RH^§. K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. Mol. Cell. pii: S1097-2765(14)00268-8. 2014.

Kuo JC^§. Mechanotransduction at focal adhesions: integrating cytoskeletal mechanics in migrating cells. J Cell Mol Med. 17(6):704-712. 2013.

Chen DY, Li MY, Wu SY, Lin YL, Tsai SP, Lai PL, Lin YT, Kuo JC, Meng TC, Chen GC^§. The Bro1 domain-containing Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration. J Cell Sci. 125(pt20): 4841-52. 2012.

Kuo JC, Han X, Yates JR, Waterman CM^§. Isolation of focal adhesion proteins for biochemical and proteomic analysis. Method Mol. Biology (Integrin and Cell Adhesion Molecules). 757:297-323. 2012.

Li CC, Kuo JC, Kiyama R, Moss J, Vaughan M^§. Brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 1 and KANK1 participate in modulating cell polarity and directed migration during wound healing. PNAS. 108(48):19228-33. 2011.

Valdes JL, Tang J, Mcdermott MI, Kuo JC, Zimmerman SP, Wincovitch SM, Waterman CM, Milgram SL, Playford MP^§. Sorting Nexin 27 protein regulates trafficking of a p21-activated kinase (PAK) interacting exchange factor (β-Pix)-G protein-coupled receptor kinase interacting protein (GIT) complex via a PDZ domain interaction. J. Biol. Chem. 286(45):39403-16. 2011.

Kuo JC, Han X, Hsiao CT, Yates JR, Waterman CM^§. Analysis of the myosinII-responsive focal adhesion proteome reveals a role for -Pix in negative regulation of focal adhesion maturation. Nat. Cell Biol. 13(4):383-93. 2011.* This paper was selected to be the main Nature Cell Biology April cover, and was the subject of the news and views in Nature Cell Biology (Joan S. Brugge, 13: 344, 2011) and a research highlight in Nature Reviews Molecular Cell Biology (Katrin Legg, 12:278, 2011).

Wang WJ, Kuo JC, Ku W, Lee YR, Lin FC, Chang YL, Lin YM, Chen CH, Huang YP, Chiang MJ, Yeh SW, Wu PR, Shen CH, Wu CT, and Chen RH^§. The tumor suppressor DAPK is reciprocally regulated by tyrosine kinase Src and phosphatase LAR. Mol. Cell. 27(5):701-16. 2007.

Kuo JC, Wang WJ, Yao CC, Wu PR, and Chen RH^§. The tumor suppressor DAPK inhibits cell motility by blocking integrin-mediated polarity pathway. J Cell Biol. 172(4): 619-31. 2006.

Chen RH^§, Wang WJ, and Kuo JC. The tumor suppressor DAP-kinase links cell adhesion and cytoskeleton reorganization to cell death regulation. J Biomed Sci. 3: 1-7. 2006.

Chen CH, Wang WJ, Kuo JC, Tsai HC, Lin JR, Chang ZF, Chen RH^§. Bidirectional signals transduced by DAPK-ERK interaction promote the apoptotic effect of DAPK. EMBO J. 24: 294-304. 2005.

Kuo JC, Lin JR, Staddon JM, Hosoya H, Chen RH^§. Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase. J Cell Sci. 116: 4777-4790. 2003.

Wang WJ, Kuo JC, Yao CC, Chen RH^§. DAP-kinase induces apoptosis by suppressing integrin activity and disrupting matrix survival signals. J Cell Biol. 159: 169-79. 2002.